Procoat, the precursor of M13 coat protein, inserts post-translationally into the membrane of cells infected by wild-type virus.
نویسندگان
چکیده
In growing cells infected by wild-type coliphage M13, the synthesis of procoat protein is completed before it inserts into the plasma membrane ane is converted to coat protein.
منابع مشابه
Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.
The major coat protein (gene 8 product) of coliphage M13 is an integral protein of the host cell cytoplasmic membrane prior to virus assembly. It is synthesized as a precursor, termed procoat, with an extra 23 NH2terminal residues. We have studied the synthesis, assembly, and processing of procoat protein by amino acid pulse-labeling E. coli which are infected by either M13 or by M13 with amber...
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Procoat, the precursor form of the major coat protein of coliphage M13, assembles into the Escherichia coli inner membrane and is cleaved to mature coat protein by leader peptidase. This assembly process has previously been reconstituted using lipids and purified leader peptidase in a cell-free protein synthesis reaction (Watts, C., Silver, P., and Wickner, W. (1981) Cell 25, 347-353; Ohno-Iwas...
متن کاملCysteine residues in the transmembrane regions of M13 procoat protein suggest that oligomeric coat proteins assemble onto phage progeny.
The M13 phage assembles in the inner membrane of Escherichia coli. During maturation, about 2,700 copies of the major coat protein move from the membrane onto a single-stranded phage DNA molecule that extrudes out of the cell. The major coat protein is synthesized as a precursor, termed procoat protein, and inserts into the membrane via a Sec-independent pathway. It is processed by a leader pep...
متن کاملCysteine residues in the transmembrane regions of M13 procoat suggest that oligomeric coat proteins assemble onto phage progeny
1 2 The M13 phage assembles in the inner membrane of Escherichia coli. During 3 maturation, about 2700 copies of the major coat protein move from the membrane 4 onto a single-stranded phage DNA molecule that extrudes out of the cell. The major 5 coat protein is synthesized as a precursor, termed procoat protein and inserts into 6 the membrane via a Sec-independent pathway. It is processed by le...
متن کاملIsolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo.
The only covalent modifications known to accompany protein insertion into membranes or protein secretion are glycosylation and the proteolytic removal of an NH2-terminal leader (signal) sequence. This latter reaction is catalyzed by leader peptidase, a constitutive, membrane-bound proteinase. We now report the identification of a plasmid-bearing strain of Escherichia coli that overproduces lead...
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ورودعنوان ژورنال:
- Journal of virology
دوره 37 3 شماره
صفحات -
تاریخ انتشار 1981